| Chapter title |
Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot-Conjugated Proteins.
|
|---|---|
| Chapter number | 20 |
| Book title |
Semaphorin Signaling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6448-2_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6446-8, 978-1-4939-6448-2
|
| Authors |
Ioana Carcea, Deanna L. Benson |
| Editors |
Jonathan R. Terman |
| Abstract |
Neurons can endocytose soluble semaphorins to either initiate or interrupt signaling at the cell membrane. Depending on the cell type and even on the specific subcellular domain, the endocytic process will differ in intensity, speed, and modality, and will subsequently facilitate diverse actions of semaphorin molecules. Therefore, in order to understand the physiology of guidance cues like semaphorins it is important to visualize endocytic events with good spatial and temporal resolution. Here, we describe methods to visualize endocytosed Semaphorin3A (Sema3A) molecules and to characterize the rate and pathway of internalization in primary rat neuronal cultures using semiconductor quantum dot nanoparticles (Q-dots). |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 33% |
| Unknown | 2 | 67% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 1 | 33% |
| Unknown | 2 | 67% |