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Semaphorin Signaling

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Cover of 'Semaphorin Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Semaphorins and their Signaling Mechanisms.
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    Chapter 2 Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells.
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    Chapter 3 Expression and Purification of Class 7 Semaphorin and Its PlexinC1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction.
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    Chapter 4 Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System.
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    Chapter 5 Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions.
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    Chapter 6 Characterizing Plexin GTPase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry, and Isothermal Titration Calorimetry.
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    Chapter 7 In Vitro Assay for the Rap GTPase-Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin.
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    Chapter 8 Characterizing F-actin Disassembly Induced by the Semaphorin-Signaling Component MICAL.
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    Chapter 9 Characterizing ErbB-2-Mediated Tyrosine Phosphorylation and Activation of Plexins.
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    Chapter 10 Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins.
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    Chapter 11 Using Heterologous COS-7 Cells to Identify Semaphorin-Signaling Components.
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    Chapter 12 Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition.
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    Chapter 13 Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse.
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    Chapter 14 An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse.
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    Chapter 15 Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins: A Primary Culture Approach.
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    Chapter 16 Semaphorin Signaling
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    Chapter 17 Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues.
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    Chapter 18 Assays to Examine Transmembrane Semaphorin Function In Vitro.
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    Chapter 19 Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth.
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    Chapter 20 Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot-Conjugated Proteins.
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    Chapter 21 Visualization of Clathrin-Mediated Endocytosis During Semaphorin-Guided Axonal Growth.
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    Chapter 22 Semaphorin Signaling
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    Chapter 23 Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors.
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    Chapter 24 Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development.
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    Chapter 25 Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis Through Regulation of VEGF Signaling.
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    Chapter 26 Semaphorin Signaling
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    Chapter 27 Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay.
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    Chapter 28 Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.
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    Chapter 29 In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling.
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    Chapter 30 Semaphorin Signaling
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    Chapter 31 Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.
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    Chapter 32 Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity During Embryonic Development.
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    Chapter 33 Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models.
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    Chapter 34 Characterizing Semaphorin Signaling In Vivo Using C. elegans.
Attention for Chapter 34: Characterizing Semaphorin Signaling In Vivo Using C. elegans.
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Chapter title
Characterizing Semaphorin Signaling In Vivo Using C. elegans.
Chapter number 34
Book title
Semaphorin Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6448-2_34
Pubmed ID
Book ISBNs
978-1-4939-6446-8, 978-1-4939-6448-2
Authors

Akira Nukazuka, Shin Takagi

Editors

Jonathan R. Terman

Abstract

A small model animal Caenorhabditis elegans is particularly suitable for genetic analysis, but cell-type-specific biochemistry is a formidable task in this organism. Here we describe techniques utilizing transgenic C. elegans strains expressing epitope-tagged proteins for analyzing biochemical events, such as protein phosphorylation and formation of protein complex, in a small number of a specific group of cells at a defined stage of development. The techniques are useful for elucidating that C. elegans semaphorin-plexin signaling systems regulate epidermal morphogenesis through modulating TOR signaling and its downstream targets.

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The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 50%
Student > Ph. D. Student 1 50%
Other 1 50%
Readers by discipline Count As %
Unspecified 1 50%
Neuroscience 1 50%
Medicine and Dentistry 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2018.
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#20,353,668
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Outputs from Methods in molecular biology
#9,922
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#355,334
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Outputs of similar age from Methods in molecular biology
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