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ERK Signaling

Overview of attention for book
Cover of 'ERK Signaling'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 How Genetics Has Helped Piece Together the MAPK Signaling Pathway.
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    Chapter 2 In Vitro Enzyme Kinetics Analysis of EGFR.
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    Chapter 3 High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells.
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    Chapter 4 Structural Studies of ERK2 Protein Complexes.
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    Chapter 5 Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk.
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    Chapter 6 Assaying Activation and Subcellular Localization of ERK in Cells and Tissues.
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    Chapter 7 Detection and Functional Analysis of SUMO-Modified MEK.
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    Chapter 8 Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.
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    Chapter 9 High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates.
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    Chapter 10 Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE.
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    Chapter 11 Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation.
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    Chapter 12 Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions.
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    Chapter 13 The Nuclear Translocation of ERK.
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    Chapter 14 Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).
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    Chapter 15 Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors.
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    Chapter 16 Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers.
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    Chapter 17 Co-culture Activation of MAP Kinase in Drosophila S2 Cells.
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    Chapter 18 ERK Signaling
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    Chapter 19 3D Organotypic Culture Model to Study Components of ERK Signaling.
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    Chapter 20 Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway.
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    Chapter 21 Genome-Wide Analysis of RAS/ERK Signaling Targets.
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    Chapter 22 Probing Chromatin Modifications in Response to ERK Signaling.
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    Chapter 23 Analyzing pERK Activation During Planarian Regeneration.
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    Chapter 24 Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development.
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    Chapter 25 Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images.
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    Chapter 26 Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.
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    Chapter 27 Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis.
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    Chapter 28 Modeling RASopathies with Genetically Modified Mouse Models.
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    Chapter 29 Dissecting Cell-Fate Determination Through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.
Attention for Chapter 18: ERK Signaling
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Chapter title
ERK Signaling
Chapter number 18
Book title
ERK Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6424-6_18
Pubmed ID
27924572
Book ISBNs
978-1-4939-6422-2, 978-1-4939-6424-6
Authors

Saba-El-Leil, Marc K, Frémin, Christophe, Meloche, Sylvain, Marc K. Saba-El-Leil, Christophe Frémin, Sylvain Meloche

Editors

Gerardo Jimenez

Abstract

Mouse embryonic stem (ES) cells have proven to be invaluable research tools for dissecting the role of signaling pathways in embryonic development, adult physiology, and various diseases. ES cells are amenable to genetic manipulation by classical gene targeting via homologous recombination or by genome editing technologies. These cells can be used to generate genetically modified mouse models or to study the signaling circuitry regulating self-renewal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.

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Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2016.
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#20,359,475
of 22,908,162 outputs
Outputs from Methods in molecular biology
#9,921
of 13,131 outputs
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#355,349
of 420,477 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,074 outputs
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