| Chapter title |
Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis.
|
|---|---|
| Chapter number | 27 |
| Book title |
ERK Signaling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6424-6_27 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6422-2, 978-1-4939-6424-6
|
| Authors |
Takaaki Matsui, Yasumasa Bessho |
| Editors |
Gerardo Jimenez |
| Abstract |
During vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis. |
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