| Chapter title |
3D Organotypic Culture Model to Study Components of ERK Signaling.
|
|---|---|
| Chapter number | 19 |
| Book title |
ERK Signaling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6424-6_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6422-2, 978-1-4939-6424-6
|
| Authors |
Athina-Myrto Chioni, Rabia Tayba Bajwa, Richard Grose |
| Editors |
Gerardo Jimenez |
| Abstract |
Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic the structure and function of cells and tissues found inside the body. Methods for developing 3D organotypic models differ according to the tissue of interest and the experimental design. For example, cultures may be grown submerged in culture medium and or at an air-liquid interface. Our group is focusing on an air-liquid interface 3D organotypic model. These cultures are grown on a nylon membrane-covered metal grid with the cells embedded in a Collagen-Matrigel gel. This allows cells to grow in an air-liquid interface to enable diffusion and nourishment from the medium below. Subsequently, the organotypic cultures can be used for immunohistochemical staining of various components of ERK signaling, which is a key player in mediating communication between cells and their microenvironment. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 42% |
| Student > Ph. D. Student | 2 | 17% |
| Other | 1 | 8% |
| Student > Master | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Neuroscience | 1 | 8% |
| Chemistry | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 4 | 33% |