| Chapter title |
Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications
|
|---|---|
| Chapter number | 18 |
| Book title |
Hepatocyte Transplantation
|
| Published in |
Methods in molecular biology, November 2016
|
| DOI | 10.1007/978-1-4939-6506-9_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6504-5, 978-1-4939-6506-9
|
| Authors |
Raphael P. H. Meier, Elisa Montanari, Philippe Morel, Joël Pimenta, Henk-Jan Schuurman, Christine Wandrey, Sandrine Gerber-Lemaire, Redouan Mahou, Leo H. Bühler |
| Editors |
Peggy Stock, Bruno Christ |
| Abstract |
Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 15% |
| Other | 2 | 8% |
| Student > Postgraduate | 2 | 8% |
| Researcher | 2 | 8% |
| Student > Ph. D. Student | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 12 | 46% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 27% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Immunology and Microbiology | 1 | 4% |
| Agricultural and Biological Sciences | 1 | 4% |
| Unknown | 13 | 50% |