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Mendeley readers
Chapter title |
A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver
|
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Chapter number | 4 |
Book title |
Hepatocyte Transplantation
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Published in |
Methods in molecular biology, November 2016
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DOI | 10.1007/978-1-4939-6506-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6504-5, 978-1-4939-6506-9
|
Authors |
David C. Bartlett, Philip N. Newsome |
Editors |
Peggy Stock, Bruno Christ |
Abstract |
Successful hepatocyte isolation is critical for continued development of cellular transplantation. However, most tissue available for research is from diseased liver and the results of hepatocyte isolation from such tissue are inferior compared to normal tissue. Here we describe a modified method, combining the use of Liberase and N-acetylcysteine (NAC), for the isolation of primary human hepatocytes with high viability from normal and diseased liver. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 40% |
Student > Ph. D. Student | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 40% |
Agricultural and Biological Sciences | 1 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Unknown | 1 | 20% |