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Mendeley readers
| Chapter title |
High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes
|
|---|---|
| Chapter number | 4 |
| Book title |
Haplotyping
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6750-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6748-3, 978-1-4939-6750-6
|
| Authors |
Nicholas M. Murphy, Colin W. Pouton, Helen R. Irving |
| Editors |
Irene Tiemann-Boege, Andrea Betancourt |
| Abstract |
Here, we describe how peptide nucleic acid (PNA) probes can be used to enrich genomic DNA fractions to facilitate downstream analysis, such as the haplotype phasing of the isolated genomic pieces. This method enriches for polymorphic regions of fragmented chromosomes by physically separating the desired sequence and flanking regions. The PNA probes used for enrichment are novel synthetic nucleic acids with highly specific targeting and hybridization properties. Using a enrichment technique, we capture high molecular weight genomic DNA using nothing more than a simple modification to standard genomic DNA extraction from blood. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 40% |
| Professor | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
| Engineering | 1 | 20% |
| Unknown | 1 | 20% |