Chapter title |
Manipulating Cholesterol Status Within Cells
|
---|---|
Chapter number | 4 |
Book title |
Cholesterol Homeostasis
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6875-6_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6873-2, 978-1-4939-6875-6
|
Authors |
Winnie Luu Ph.D., Ingrid C. Gelissen Ph.D., Andrew J. Brown Ph.D., Luu, Winnie, Gelissen, Ingrid C., Brown, Andrew J., Winnie Luu, Ingrid C. Gelissen, Andrew J. Brown |
Editors |
Ingrid C. Gelissen, Andrew J. Brown |
Abstract |
Cellular cholesterol levels are intricately controlled to maintain homeostasis. Here, we describe ways in which cellular cholesterol status can be manipulated for the study of cholesterol homeostasis, including sterol starvation (by culturing cells in lipoprotein-deficient serum and pretreating/treating with the cholesterol-lowering drug, statin) and sterol enrichment (using cholesterol complexed to cyclodextrin, and low-density lipoprotein). We also describe how to prepare lipoprotein-deficient serum and complex cholesterol to cyclodextrin. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 6% |
Netherlands | 1 | 6% |
Unknown | 15 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 35% |
Researcher | 5 | 29% |
Student > Master | 3 | 18% |
Student > Bachelor | 2 | 12% |
Professor > Associate Professor | 1 | 6% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 53% |
Medicine and Dentistry | 3 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Computer Science | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 0 | 0% |
Unknown | 2 | 12% |