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Cholesterol Homeostasis

Overview of attention for book
Cover of 'Cholesterol Homeostasis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Overview of Cholesterol Homeostasis
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    Chapter 2 Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins
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    Chapter 3 Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review
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    Chapter 4 Manipulating Cholesterol Status Within Cells
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    Chapter 5 Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells
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    Chapter 6 The Use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolemia and Atherosclerosis
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    Chapter 7 CRISPR/Cas9-Mediated Generation of Niemann–Pick C1 Knockout Cell Line
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    Chapter 8 Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*
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    Chapter 9 Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
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    Chapter 10 Synthesis and Live-Cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport
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    Chapter 11 Measurement of Cholesterol Transfer from Lysosome to Peroxisome Using an In Vitro Reconstitution Assay
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    Chapter 12 Measurement of Mitochondrial Cholesterol Import Using a Mitochondria-Targeted CYP11A1 Fusion Construct
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    Chapter 13 Identifying Sterol Response Elements Within Promoters of Genes
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    Chapter 14 Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion
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    Chapter 15 Determining the Topology of Membrane-Bound Proteins Using PEGylation
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    Chapter 16 Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry
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    Chapter 17 Sterol Analysis by Quantitative Mass Spectrometry
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    Chapter 18 Measurement of Rates of Cholesterol and Fatty Acid Synthesis In Vivo Using Tritiated Water
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    Chapter 19 Methods for Monitoring ABCA1-Dependent Sterol Release
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    Chapter 20 ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols
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    Chapter 21 Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
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    Chapter 22 Erratum to: Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
Attention for Chapter 8: Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*
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Chapter title
Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*
Chapter number 8
Book title
Cholesterol Homeostasis
Published in
Methods in molecular biology, February 2017
DOI 10.1007/978-1-4939-6875-6_8
Pubmed ID
28205169
Book ISBNs
978-1-4939-6873-2, 978-1-4939-6875-6
Authors

Jian Li, Peter L. Lee, Suzanne R. Pfeffer

Editors

Ingrid C. Gelissen, Andrew J. Brown

Abstract

Methods to quantify intracellular cholesterol are valuable for the study of its trafficking and storage in normal cells and in lysosomal storage disorders. Traditionally, cholesterol has been tracked using the small molecule, filipin. Filipin can be difficult to visualize and visualization can be cytotoxic as it requires UV illumination. Here we describe a method to measure cholesterol using a fluorescently labeled, mutant form of Perfringolysin O, a soluble protein toxin that binds cholesterol specifically. This approach has been used to measure the impact of NPC1 deficiency on lysosomal cholesterol levels and monitor the rescue of cholesterol export under conditions that reduce the thickness of the lysosomal glycocalyx.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 18%
Researcher 2 18%
Student > Ph. D. Student 2 18%
Unspecified 1 9%
Other 1 9%
Other 2 18%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Engineering 2 18%
Unspecified 1 9%
Arts and Humanities 1 9%
Decision Sciences 1 9%
Other 1 9%
Unknown 1 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2018.
All research outputs
#17,876,644
of 22,953,506 outputs
Outputs from Methods in molecular biology
#7,259
of 13,137 outputs
Outputs of similar age
#222,072
of 307,002 outputs
Outputs of similar age from Methods in molecular biology
#132
of 253 outputs
Altmetric has tracked 22,953,506 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,002 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 253 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.

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