↓ Skip to main content

Cholesterol Homeostasis

Overview of attention for book
Cover of 'Cholesterol Homeostasis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Overview of Cholesterol Homeostasis
  3. Altmetric Badge
    Chapter 2 Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins
  4. Altmetric Badge
    Chapter 3 Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review
  5. Altmetric Badge
    Chapter 4 Manipulating Cholesterol Status Within Cells
  6. Altmetric Badge
    Chapter 5 Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells
  7. Altmetric Badge
    Chapter 6 The Use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolemia and Atherosclerosis
  8. Altmetric Badge
    Chapter 7 CRISPR/Cas9-Mediated Generation of Niemann–Pick C1 Knockout Cell Line
  9. Altmetric Badge
    Chapter 8 Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*
  10. Altmetric Badge
    Chapter 9 Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
  11. Altmetric Badge
    Chapter 10 Synthesis and Live-Cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport
  12. Altmetric Badge
    Chapter 11 Measurement of Cholesterol Transfer from Lysosome to Peroxisome Using an In Vitro Reconstitution Assay
  13. Altmetric Badge
    Chapter 12 Measurement of Mitochondrial Cholesterol Import Using a Mitochondria-Targeted CYP11A1 Fusion Construct
  14. Altmetric Badge
    Chapter 13 Identifying Sterol Response Elements Within Promoters of Genes
  15. Altmetric Badge
    Chapter 14 Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion
  16. Altmetric Badge
    Chapter 15 Determining the Topology of Membrane-Bound Proteins Using PEGylation
  17. Altmetric Badge
    Chapter 16 Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry
  18. Altmetric Badge
    Chapter 17 Sterol Analysis by Quantitative Mass Spectrometry
  19. Altmetric Badge
    Chapter 18 Measurement of Rates of Cholesterol and Fatty Acid Synthesis In Vivo Using Tritiated Water
  20. Altmetric Badge
    Chapter 19 Methods for Monitoring ABCA1-Dependent Sterol Release
  21. Altmetric Badge
    Chapter 20 ABC-Transporter Mediated Sterol Export from Cells Using Radiolabeled Sterols
  22. Altmetric Badge
    Chapter 21 Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
  23. Altmetric Badge
    Chapter 22 Erratum to: Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice
Attention for Chapter 9: Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
6 Dimensions

Readers on

mendeley
1 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
Chapter number 9
Book title
Cholesterol Homeostasis
Published in
Methods in molecular biology, February 2017
DOI 10.1007/978-1-4939-6875-6_9
Pubmed ID
Book ISBNs
978-1-4939-6873-2, 978-1-4939-6875-6
Authors

Leslie A. McCauliff, Judith Storch

Editors

Ingrid C. Gelissen, Andrew J. Brown

Abstract

In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of protein-membrane interactions that may occur during cholesterol transfer by simply modifying donor or acceptor concentrations, membrane composition, or buffer properties, and analyzing resultant transfer rates.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 February 2017.
All research outputs
#18,531,724
of 22,953,506 outputs
Outputs from Methods in molecular biology
#7,935
of 13,137 outputs
Outputs of similar age
#235,458
of 307,002 outputs
Outputs of similar age from Methods in molecular biology
#148
of 253 outputs
Altmetric has tracked 22,953,506 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,002 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 253 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.