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Plant Pattern Recognition Receptors

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Cover of 'Plant Pattern Recognition Receptors'

Table of Contents

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    Book Overview
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    Chapter 1 Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors
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    Chapter 2 Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses
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    Chapter 3 Methods of Isolation and Characterization of Oligogalacturonide Elicitors
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    Chapter 4 Quantitative Analysis of Ligand-Induced Endocytosis of FLAGELLIN-SENSING 2 Using Automated Image Segmentation
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    Chapter 5 Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants
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    Chapter 6 Assays to Investigate the N-Glycosylation State and Function of Plant Pattern Recognition Receptors
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    Chapter 7 Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions
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    Chapter 8 In Vitro Ubiquitination Activity Assays in Plant Immune Responses
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    Chapter 9 Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily
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    Chapter 10 Identification of MAPK Substrates Using Quantitative Phosphoproteomics
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    Chapter 11 Analysis of PAMP-Triggered ROS Burst in Plant Immunity
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    Chapter 12 MAPK Assays in Arabidopsis MAMP-PRR Signal Transduction
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    Chapter 13 LeEIX2 Interactors’ Analysis and EIX-Mediated Responses Measurement
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    Chapter 14 CDPK Activation in PRR Signaling
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    Chapter 15 Chitin and Stress Induced Protein Kinase Activation
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    Chapter 16 Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, During Bacterial Infection in Arabidopsis
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    Chapter 17 Quantitative Evaluation of Plant Actin Cytoskeletal Organization During Immune Signaling
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    Chapter 18 Network Reconstitution for Quantitative Subnetwork Interaction Analysis
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    Chapter 19 Stomatal Bioassay to Characterize Bacterial-Stimulated PTI at the Pre-Invasion Phase of Infection
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    Chapter 20 Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response
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    Chapter 21 Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato
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    Chapter 22 Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae
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    Chapter 23 Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment
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    Chapter 24 Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants
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    Chapter 25 Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing
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    Chapter 26 Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells
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    Chapter 27 Chitin-Induced Responses in the Moss Physcomitrella patens
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    Chapter 28 Methods to Quantify PAMP-Triggered Oxidative Burst, MAP Kinase Phosphorylation, Gene Expression, and Lignification in Brassicas
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    Chapter 29 Effectoromics-Based Identification of Cell Surface Receptors in Potato
Attention for Chapter 15: Chitin and Stress Induced Protein Kinase Activation
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Chapter title
Chitin and Stress Induced Protein Kinase Activation
Chapter number 15
Book title
Plant Pattern Recognition Receptors
Published in
Methods in molecular biology, February 2017
DOI 10.1007/978-1-4939-6859-6_15
Pubmed ID
28220425
Book ISBNs
978-1-4939-6858-9, 978-1-4939-6859-6
Authors

Chandra Kenchappa, Raquel Azevedo da Silva, Simon Bressendorff, Sabrina Stanimirovic, Jakob Olsen, Morten Petersen, John Mundy

Editors

Libo Shan, Ping He

Abstract

The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

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The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 100%
Readers by discipline Count As %
Environmental Science 1 100%

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