| Chapter title |
Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment
|
|---|---|
| Chapter number | 23 |
| Book title |
Plant Pattern Recognition Receptors
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6859-6_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
| Authors |
Yingnan Hou, Wenbo Ma |
| Editors |
Libo Shan, Ping He |
| Abstract |
Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Master | 1 | 17% |
| Researcher | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Unknown | 1 | 17% |