Chapter title |
A Mini-Electrochemical System with Integrated Micropipet Tip and Pencil Graphite Electrode for Measuring Cytotoxicity
|
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Chapter number | 11 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Dong-Mei Wu, Xiao-Ling Guo, Qian Wang, Jin-Lian Li, Ji-Wen Cui, Shi Zhou, Su-E Hao |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
A novel mini-electrochemical system has been developed for evaluating cytotoxicity of anticancer drugs based on trace cell samples. The mini-electrochemical system was integrated by using pencil graphite modified with threonine as working electrode, an Ag/AgCl reference electrode and a micropipet tip as electrochemical cell. The mini-electrochemical system dramatically reduces sample volumes from 500 μL in a traditional electrochemical system to 10 μL, and exhibits excellent electrocatalytic activity toward oxidation of purine from MCF-7 cells due to increased sensitivity provided by threonine. Moreover, the relationship between peak current and the cell concentration in the range from 3.0 × l0(3) to 7.0 × l0(6) cells/mL was studied, and a nonlinear exponential relationship between them was established over a wide concentration range. In evaluating the effect of anticancer drugs on cell viability, the results of drug cytotoxicity test based on cyclophosphamide were in close agreement with classical 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. The proposed device is so simple, cheap, and easy to operate that it could be applied to single-use applications. The mini-electrochemical system proved to be a useful tool and can be applied to electrochemical studies of cancer cells as well as other biological samples such as proteins and DNA. |
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