Chapter title |
A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms
|
---|---|
Chapter number | 27 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Lingwen Zeng, Zhuo Xiao |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step 2, the reaction product of step 1 is detected by a lateral flow biosensor, which is a rapid and cost effective tool for nuclei acid detection. Comparing with conventional methods, it requires no complicated machines. It is suitable for the use of point of care diagnostics. Therefore, this simple, cost effective, robust, and promising LFB detection method of SNP has great potential for the detection of genetic diseases, personalized medicine, cancer related mutations, and drug-resistant mutations of infectious agents. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 90 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 19 | 21% |
Student > Master | 14 | 16% |
Researcher | 12 | 13% |
Student > Bachelor | 7 | 8% |
Student > Doctoral Student | 4 | 4% |
Other | 11 | 12% |
Unknown | 23 | 26% |
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Biochemistry, Genetics and Molecular Biology | 13 | 14% |
Agricultural and Biological Sciences | 9 | 10% |
Engineering | 9 | 10% |
Materials Science | 8 | 9% |
Chemical Engineering | 6 | 7% |
Other | 20 | 22% |
Unknown | 25 | 28% |