Chapter title |
Analysis of Chromatin Dynamics During Drosophila Spermatogenesis
|
---|---|
Chapter number | 17 |
Book title |
Meiosis
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6340-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6338-6, 978-1-4939-6340-9
|
Authors |
Hundertmark, Tim, Theofel, Ina, Eren-Ghiani, Zeynep, Miller, David, Rathke, Christina, Tim Hundertmark, Ina Theofel, Zeynep Eren-Ghiani, David Miller, Christina Rathke |
Abstract |
In the course of spermatogenesis, germ cells undergo dramatic morphological changes that affect almost all cellular components. Therefore, it is impossible to study the process of spermatogenesis in its entirety without detailed morphological analyses. Here, we describe a method to visualize chromatin dynamics in differentiating Drosophila male germ cells using immunofluorescence staining. In addition, we demonstrate how to treat Drosophila sperm before immunofluorescence staining to help reveal epitopes in the highly condensed sperm chromatin that otherwise may be inaccessible to antibodies. |
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