Chapter title |
CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry
|
---|---|
Chapter number | 14 |
Book title |
Protein-Carbohydrate Interactions
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6899-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6898-5, 978-1-4939-6899-2
|
Authors |
Badruna, Louise, Burlat, Vincent, Montanier, Cédric Y., Louise Badruna, Vincent Burlat, Cédric Y. Montanier |
Editors |
D. Wade Abbott, Alicia Lammerts van Bueren |
Abstract |
Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated polysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction. |
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