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Mendeley readers
| Chapter title |
Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay
|
|---|---|
| Chapter number | 3 |
| Book title |
Protein-Carbohydrate Interactions
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6899-2_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6898-5, 978-1-4939-6899-2
|
| Authors |
Lauren S. McKee |
| Editors |
D. Wade Abbott, Alicia Lammerts van Bueren |
| Abstract |
Use of the 3,5-dinitrosalicylic acid reagent allows the simple and rapid quantification of reducing sugars. The method can be used for analysis of biological samples or in the characterization of enzyme reactions. Presented here is an application of the method in measuring the kinetics of a glycoside hydrolase reaction, including the optimization of the DNSA reagent, and the production of a standard curve of absorbance and sugar concentration. |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 9 | 26% |
| Student > Master | 4 | 11% |
| Researcher | 3 | 9% |
| Lecturer | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 17 | 49% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 20% |
| Agricultural and Biological Sciences | 2 | 6% |
| Chemistry | 2 | 6% |
| Unspecified | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 4 | 11% |
| Unknown | 18 | 51% |