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Mendeley readers
Chapter title |
A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases
|
---|---|
Chapter number | 1 |
Book title |
Protein-Carbohydrate Interactions
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6899-2_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6898-5, 978-1-4939-6899-2
|
Authors |
Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, Harry Brumer |
Editors |
D. Wade Abbott, Alicia Lammerts van Bueren |
Abstract |
The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of glycoside hydrolase (GH) activity, particularly on soluble polysaccharide substrates. Here, we describe a straightforward method adapted to low-volume polymerase chain reaction (PCR) tubes which enables the rapid, parallel determination of GH kinetics in applications ranging from initial activity screening and assay optimization, to precise Michaelis-Menten analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 23% |
Student > Bachelor | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Researcher | 2 | 15% |
Lecturer > Senior Lecturer | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 38% |
Chemistry | 2 | 15% |
Immunology and Microbiology | 2 | 15% |
Agricultural and Biological Sciences | 1 | 8% |
Unknown | 3 | 23% |