| Chapter title |
Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial C... - PubMed - NCBI
|
|---|---|
| Chapter number | 16 |
| Book title |
PCR
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7060-5_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
| Authors |
Phadke, Sujal, Salvador, Andreia Filipa, Alves, Joana Isabel, Bretschger, Orianna, Alves, Maria Madalena, Pereira, Maria Alcina, Sujal Phadke, Andreia Filipa Salvador, Joana Isabel Alves, Orianna Bretschger, Maria Madalena Alves, Maria Alcina Pereira |
| Editors |
Lucília Domingues |
| Abstract |
Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 30% |
| Professor | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 40% |
| Environmental Science | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Engineering | 1 | 10% |
| Unknown | 3 | 30% |