You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Long Fragment Polymerase Chain Reaction.
|
|---|---|
| Chapter number | 3 |
| Book title |
PCR
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7060-5_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
| Authors |
Eng Wee Chua, Simran Maggo, Martin A. Kennedy |
| Editors |
Lucília Domingues |
| Abstract |
Polymerase chain reaction (PCR) is an oft-used preparatory technique in amplifying specific DNA regions for downstream analysis. The size of an amplicon was initially limited by errors in nucleotide polymerization and template deterioration during thermal cycling. A variant of PCR, designated long-range PCR, was devised to counter these drawbacks and enable the amplification of large fragments exceeding a few kb. In this chapter we describe a protocol for long-range PCR, which we have adopted to obtain products of 6.6, 7.2, 13, and 20 kb from human genomic DNA samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 16% |
| Other | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Student > Postgraduate | 3 | 10% |
| Student > Ph. D. Student | 3 | 10% |
| Other | 5 | 16% |
| Unknown | 9 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 29% |
| Agricultural and Biological Sciences | 7 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
| Unspecified | 1 | 3% |
| Computer Science | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 8 | 26% |