| Chapter title |
Multiplex Detection of Food-Borne Pathogens
|
|---|---|
| Chapter number | 10 |
| Book title |
PCR
|
| Published in |
Methods in molecular biology, May 2017
|
| DOI | 10.1007/978-1-4939-7060-5_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
| Authors |
Germán Villamizar-Rodríguez, Felipe Lombó |
| Editors |
Lucília Domingues |
| Abstract |
Detection of food-borne pathogens is traditionally carried out by plating out techniques in selective or differential media using Petri agar dishes or other culture-dependent methods, usually designed for each pathogen to be detected. These classical methods are time and personnel consuming and also may last for up to 5 days in the case of final confirmation of some specific pathogens.Here we describe a method for fast multiplex detection of nine food-borne pathogens (all species usually required under most countrylegislations) by means of a single multiplex PCR reaction coupled to a capillary electrophoresis detection, in just 2-2.5 h and with a minimum cost of around 2 € per sample and nine pathogens. This method saves consumables and personnel time and allows a faster detection of any possible contaminated food batches at industrial level, therefore helping to prevent future food-borne outbreaks at clinical level. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 27% |
| Student > Ph. D. Student | 2 | 9% |
| Student > Bachelor | 2 | 9% |
| Student > Master | 2 | 9% |
| Student > Doctoral Student | 1 | 5% |
| Other | 5 | 23% |
| Unknown | 4 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 18% |
| Agricultural and Biological Sciences | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Engineering | 2 | 9% |
| Immunology and Microbiology | 2 | 9% |
| Other | 1 | 5% |
| Unknown | 8 | 36% |