Chapter title |
Efficient Quantitative Comparisons of Plasma Proteomes Using Label-Free Analysis with MaxQuant
|
---|---|
Chapter number | 23 |
Book title |
Serum/Plasma Proteomics
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7057-5_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7056-8, 978-1-4939-7057-5
|
Authors |
Lynn A. Beer, Pengyuan Liu, Bonnie Ky, Kurt T. Barnhart, David W. Speicher |
Editors |
David W. Greening, Richard J. Simpson |
Abstract |
Mass spectrometry (MS)-based quantitation of plasma proteomes is challenging due to the extremely wide dynamic range and molecular heterogeneity of plasma samples. However, recent advances in technology, MS instrumentation, and bioinformatics have enabled in-depth quantitative analyses of very complex proteomes, including plasma. Specifically, recent improvements in both label-based and label-free quantitation strategies have allowed highly accurate quantitative comparisons of expansive proteome datasets. Here we present a method for in-depth label-free analysis of human plasma samples using MaxQuant. |
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