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Mendeley readers
Chapter title |
Immunostaining of germline stem cells and the niche in Drosophila ovaries.
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Chapter number | 1 |
Book title |
Stem Cell Niche
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Published in |
Methods in molecular biology, January 2013
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DOI | 10.1007/978-1-62703-508-8_1 |
Pubmed ID | |
Book ISBNs |
978-1-62703-507-1, 978-1-62703-508-8
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Authors |
Lichao Luo, Phing Chian Chai, Yu Cai |
Abstract |
Stem cells have the ability to switch between proliferative (self-renewal) and differentiation modes. The Drosophila germarium is a well-established in vivo model for the study of communication between stem cells and their niche. One commonly used technique for such study is immunostaining that allows examination of protein localization at a fixed time point. This chapter provides a detailed protocol for immunofluorescence staining of Drosophila ovaries. This protocol has been optimized to enable explicit visualization of the niche structure, as well as to maximize the degree of multiplexing for protein labeling and detection. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 36% |
Student > Bachelor | 1 | 9% |
Student > Master | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 45% |
Agricultural and Biological Sciences | 4 | 36% |
Unknown | 2 | 18% |