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Stem Cell Niche

Overview of attention for book
Cover of 'Stem Cell Niche'

Table of Contents

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    Book Overview
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    Chapter 1 Immunostaining of germline stem cells and the niche in Drosophila ovaries.
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    Chapter 2 Genetic, immunofluorescence labeling, and in situ hybridization techniques in identification of stem cells in male and female germline niches.
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    Chapter 3 Visualization of adult stem cells within their niches using the Drosophila germline as a model system.
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    Chapter 4 Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates
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    Chapter 5 In Vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche.
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    Chapter 6 Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection.
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    Chapter 7 Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marrow
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    Chapter 8 Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
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    Chapter 9 Detection In Vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogenic Differentiation and Maturation of Stromal Stem Cells
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    Chapter 10 The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting
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    Chapter 11 Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minipump System
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    Chapter 12 Unbiased Stereological Method to Assess Proliferation Throughout the Subependymal Zone
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    Chapter 13 Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells
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    Chapter 14 Human and murine skeletal muscle reserve cells.
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    Chapter 15 Modulation of the host skeletal muscle niche for donor satellite cell grafting.
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    Chapter 16 Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
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    Chapter 17 Hypoxia and Visualization of the Stem Cell Niche
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    Chapter 18 Detection and isolation of human disseminated tumor cells in the murine bone marrow stem cell niche.
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    Chapter 19 Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patients with Acute Myeloid Leukemia
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    Chapter 20 Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension
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    Chapter 21 Isolation and Propagation of Colon Cancer Stem Cells
Attention for Chapter 14: Human and murine skeletal muscle reserve cells.
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Chapter title
Human and murine skeletal muscle reserve cells.
Chapter number 14
Book title
Stem Cell Niche
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-508-8_14
Pubmed ID
Book ISBNs
978-1-62703-507-1, 978-1-62703-508-8
Authors

Rana Abou-Khalil, Fabien Le Grand, Bénédicte Chazaud

Abstract

Study of stem cell phenotype and functions requires their proper isolation. Stem cells isolated from skeletal muscle are a useful tool to explore molecular pathways involved in the regulation of myogenesis. Among progenitor cells, a subset of cells, called reserve cells, has been identified, in vitro, in myogenic cell cultures. This subset of cells remains undifferentiated while the main population of progenitor cells commits to terminal myogenic differentiation. When replated, these reserve cells grow as new colonies of progenitors. At the time of differentiation, they reform both differentiated myotubes and undifferentiated reserve cells. Here, we present a protocol to obtain and further isolate reserve cells from both human and murine myogenic cell cultures, together with techniques to analyze their cell cycle status.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 42%
Student > Doctoral Student 2 17%
Researcher 2 17%
Student > Ph. D. Student 1 8%
Student > Postgraduate 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 50%
Biochemistry, Genetics and Molecular Biology 3 25%
Engineering 2 17%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2013.
All research outputs
#7,135,795
of 9,342,016 outputs
Outputs from Methods in molecular biology
#3,705
of 7,290 outputs
Outputs of similar age
#88,077
of 133,813 outputs
Outputs of similar age from Methods in molecular biology
#17
of 42 outputs
Altmetric has tracked 9,342,016 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,290 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 133,813 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.