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Chapter title |
Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling
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Chapter number | 24 |
Book title |
Extracellular Vesicles
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7253-1_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
Authors |
Simion Kreimer, Alexander R. Ivanov, Kreimer, Simion, Ivanov, Alexander R. |
Abstract |
The presented procedure allows rapid isolation of extracellular vesicles (EVs) from plasma using size-exclusion chromatography (SEC). Additionally, an approach for reducing the lipid and salt content of the EV isolate in preparation for mass spectrometry (MS)-based proteomic analysis is presented. An example setup for proteomic profiling of the processed samples by nanoflow liquid chromatography coupled to tandem mass spectrometry (nLC-MS/MS) is also presented. Approximately 1000 protein groups in blood plasma-derived EVs can be identified and quantitated following this procedure and using the described instrumentation. |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 68 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 21% |
Student > Master | 9 | 13% |
Student > Bachelor | 7 | 10% |
Student > Doctoral Student | 5 | 7% |
Researcher | 5 | 7% |
Other | 11 | 16% |
Unknown | 17 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 19 | 28% |
Chemistry | 5 | 7% |
Agricultural and Biological Sciences | 5 | 7% |
Chemical Engineering | 4 | 6% |
Medicine and Dentistry | 4 | 6% |
Other | 10 | 15% |
Unknown | 21 | 31% |