| Chapter title |
Microcapillary Chip-Based Extracellular Vesicle Profiling System
|
|---|---|
| Chapter number | 17 |
| Book title |
Extracellular Vesicles
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7253-1_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
| Authors |
Takanori Akagi, Takanori Ichiki |
| Abstract |
A microcapillary chip-based particle electrophoresis system developed for characterizing extracellular vesicles (EVs) is described. So far, it is technologically difficult to analyze or identify a heterogeneous population of particles ranging from several tens to one hundred nanometers, and hence, there is a growing demand for a new analytical method of nanoparticles among researchers working on extracellular vesicles. The analytical platform presented in this chapter allows detection of individual nanoparticles or nanovesicles of less than 50 nm in diameter and enables the characterization of nanoparticles based on multiple indexes such as concentration, diameter, zeta potential, and surface antigenicity. This platform will provide a useful and easy-to-use solution for obtaining both quantitative and qualitative information on EV samples used in research and development of exosome biology and medicine. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 36% |
| Student > Doctoral Student | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Professor | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 21% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Chemistry | 2 | 14% |
| Economics, Econometrics and Finance | 1 | 7% |
| Materials Science | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 29% |