Chapter title |
Characterization of Extracellular Vesicles by Flow Cytometry
|
---|---|
Chapter number | 14 |
Book title |
Extracellular Vesicles
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7253-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
Authors |
Virginia Camacho, Vasilis Toxavidis, John C. Tigges |
Abstract |
Here, we describe a comprehensive methodology for the setup and standardization of EV analysis using nanoscale flow cytometry. Controls of different size ranges, fluorescent intensities, and materials can be used to set up distribution curves that are then used for instrument optimization and as a reference guide. Using these controls, flow cytometry instruments can be primed for the detection, analysis, and sorting of specific EV populations. This allows for cross platform comparison and the ability to monitor both quality control (QC) and quality assurance (QA). The method here describes the use of nanoparticles to optimize a flow cytometer for small particle detection. It also outlines the procedures necessary to recover EVs for downstream applications. |
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Demographic breakdown
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Researcher | 2 | 10% |
Lecturer | 1 | 5% |
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