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Protocols in In Vitro Hepatocyte Research

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Cover of 'Protocols in In Vitro Hepatocyte Research'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments
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    Chapter 2 Cryopreservation of Hepatocytes
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    Chapter 3 Protocols in In Vitro Hepatocyte Research
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    Chapter 4 Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
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    Chapter 5 Culture and Functional Characterization of Human Hepatoma HepG2 Cells
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    Chapter 6 Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
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    Chapter 7 Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes
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    Chapter 8 Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation
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    Chapter 9 Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling.
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    Chapter 10 Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation
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    Chapter 11 Coculture and Long-Term Maintenance of Hepatocytes.
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    Chapter 12 Primary Hepatocytes in Sandwich Culture
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    Chapter 13 Establishing Liver Bioreactors for In Vitro Research
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    Chapter 14 Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture.
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    Chapter 15 Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors
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    Chapter 16 Transcriptomics of Hepatocytes Treated with Toxicants for Investigating Molecular Mechanisms Underlying Hepatotoxicity.
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    Chapter 17 Global MicroRNA Analysis in Primary Hepatocyte Cultures.
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    Chapter 18 Mass Spectrometry-Based Proteomics for Relative Protein Quantification and Biomarker Identification in Primary Human Hepatocytes
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    Chapter 19 Targeted Metabolomics for Homocysteine-Related Metabolites in Primary Hepatocytes
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    Chapter 20 Measurement of Cytochrome P450 Enzyme Induction and Inhibition in Human Hepatoma Cells
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    Chapter 21 Analysis of Sinusoidal Drug Uptake Transporter Activities in Primary Human Hepatocytes
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    Chapter 22 Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures
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    Chapter 23 Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes
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    Chapter 24 Functionality Testing of Primary Hepatocytes in Culture by Measuring Urea Synthesis
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    Chapter 25 Protocols in In Vitro Hepatocyte Research
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    Chapter 26 General Cytotoxicity Assessment by Means of the MTT Assay
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    Chapter 27 Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures
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    Chapter 28 Critical Factors in the Assessment of Cholestatic Liver Injury In Vitro
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    Chapter 29 In Vitro Cell Culture Models of Hepatic Steatosis
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    Chapter 30 Assessment of Liver Fibrotic Insults In Vitro
Attention for Chapter 22: Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures
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Chapter title
Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures
Chapter number 22
Book title
Protocols in In Vitro Hepatocyte Research
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2074-7_22
Pubmed ID
26272152
Book ISBNs
978-1-4939-2073-0, 978-1-4939-2074-7
Authors

Karolien Buyl, Joery De Kock, Jennifer Bolleyn, Vera Rogiers, Tamara Vanhaecke, Buyl, Karolien, Kock, Joery De, Bolleyn, Jennifer, Rogiers, Vera, Vanhaecke, Tamara

Abstract

One of the most important functions of hepatocytes is the synthesis of serum proteins, more specifically of serum albumin. Albumin secretion in serum is essential, since it maintains the oncotic pressure in the body. Measurement of albumin secretion is used as a liver function test to indicate potential liver injury and liver pathology. In this chapter, a protocol for the measurement of albumin secretion in the supernatant of cultured rat hepatocytes is described. The procedure relies on an enzyme-linked immunosorbent assay allowing rat albumin to be quantitatively measured.

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Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 17%
Student > Ph. D. Student 6 14%
Researcher 6 14%
Student > Master 6 14%
Student > Postgraduate 2 5%
Other 2 5%
Unknown 13 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 21%
Pharmacology, Toxicology and Pharmaceutical Science 6 14%
Agricultural and Biological Sciences 6 14%
Engineering 4 10%
Unspecified 1 2%
Other 2 5%
Unknown 14 33%

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