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Protocols in In Vitro Hepatocyte Research

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Cover of 'Protocols in In Vitro Hepatocyte Research'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments
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    Chapter 2 Cryopreservation of Hepatocytes
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    Chapter 3 Protocols in In Vitro Hepatocyte Research
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    Chapter 4 Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
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    Chapter 5 Culture and Functional Characterization of Human Hepatoma HepG2 Cells
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    Chapter 6 Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
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    Chapter 7 Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes
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    Chapter 8 Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation
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    Chapter 9 Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling.
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    Chapter 10 Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation
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    Chapter 11 Coculture and Long-Term Maintenance of Hepatocytes.
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    Chapter 12 Primary Hepatocytes in Sandwich Culture
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    Chapter 13 Establishing Liver Bioreactors for In Vitro Research
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    Chapter 14 Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture.
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    Chapter 15 Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors
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    Chapter 16 Transcriptomics of Hepatocytes Treated with Toxicants for Investigating Molecular Mechanisms Underlying Hepatotoxicity.
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    Chapter 17 Global MicroRNA Analysis in Primary Hepatocyte Cultures.
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    Chapter 18 Mass Spectrometry-Based Proteomics for Relative Protein Quantification and Biomarker Identification in Primary Human Hepatocytes
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    Chapter 19 Targeted Metabolomics for Homocysteine-Related Metabolites in Primary Hepatocytes
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    Chapter 20 Measurement of Cytochrome P450 Enzyme Induction and Inhibition in Human Hepatoma Cells
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    Chapter 21 Analysis of Sinusoidal Drug Uptake Transporter Activities in Primary Human Hepatocytes
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    Chapter 22 Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures
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    Chapter 23 Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes
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    Chapter 24 Functionality Testing of Primary Hepatocytes in Culture by Measuring Urea Synthesis
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    Chapter 25 Protocols in In Vitro Hepatocyte Research
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    Chapter 26 General Cytotoxicity Assessment by Means of the MTT Assay
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    Chapter 27 Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures
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    Chapter 28 Critical Factors in the Assessment of Cholestatic Liver Injury In Vitro
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    Chapter 29 In Vitro Cell Culture Models of Hepatic Steatosis
  31. Altmetric Badge
    Chapter 30 Assessment of Liver Fibrotic Insults In Vitro
Attention for Chapter 25: Protocols in In Vitro Hepatocyte Research
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Chapter title
Protocols in In Vitro Hepatocyte Research
Chapter number 25
Book title
Protocols in In Vitro Hepatocyte Research
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2074-7_25
Pubmed ID
Book ISBNs
978-1-4939-2073-0, 978-1-4939-2074-7
Authors

Johansson, Helene, Ellis, Ewa C S, Helene Johansson, Ewa C. S. Ellis, Ellis, Ewa C. S.

Abstract

Primary hepatocytes isolated from human, mouse and rat liver as well as cell lines, such as HepG2 cells, are frequently used in vitro systems in liver research. In regenerative medicine, stem cells are used for differentiation towards hepatocyte-like cells with the goal of creating differentiated functional hepatocytes. It is therefore important to measure the quality and function of highly specialized hepatocyte-specific functions using appropriate methods. In this chapter, we describe an assay to assess conjugation and excretion of labeled bile acids in cultured hepatocytes.

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Mendeley readers

Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Student > Master 2 50%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 25%
Pharmacology, Toxicology and Pharmaceutical Science 1 25%
Agricultural and Biological Sciences 1 25%
Chemistry 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2016.
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#20,325,615
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Outputs from Methods in molecular biology
#9,917
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#296,032
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Outputs of similar age from Methods in molecular biology
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