Chapter title |
In Vitro Cell Culture Models of Hepatic Steatosis
|
---|---|
Chapter number | 29 |
Book title |
Protocols in In Vitro Hepatocyte Research
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2074-7_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
Authors |
Gahl Levy, Merav Cohen, Yaakov Nahmias, Levy, Gahl, Cohen, Merav, Nahmias, Yaakov |
Abstract |
The liver is the systemic hub of lipid metabolism. The excessive accumulation of lipids in hepatocytes, steatosis, is a major clinical concern, whose progressive forms lead to end-stage liver disease. Currently, animal studies are the gold standard in toxicological risk assessment. Fueled by an integration of modern omics technologies, in silico models and in vitro system optimization, a new paradigm in the basis for toxicological risk assessment is emerging away from the use of animals. In recent years, in vitro assays have been developed for the early screening of the steatogenic potential of compounds. The present chapter describes an assay for the intracellular detection of lipids, a high-content screen for the distinction between steatosis and phospholipidosis, a multiparametric high-content screen for steatogenic potential and a liver X receptor reporter cell line. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 26% |
Student > Ph. D. Student | 6 | 22% |
Student > Master | 3 | 11% |
Student > Bachelor | 3 | 11% |
Other | 1 | 4% |
Other | 2 | 7% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 15% |
Medicine and Dentistry | 3 | 11% |
Agricultural and Biological Sciences | 2 | 7% |
Immunology and Microbiology | 1 | 4% |
Other | 3 | 11% |
Unknown | 10 | 37% |