| Chapter title |
Analyzing Genome Rearrangements in Saccharomyces cerevisiae
|
|---|---|
| Chapter number | 5 |
| Book title |
Genome Instability
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7306-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
| Authors |
Anjana Srivatsan, Christopher D. Putnam, Richard D. Kolodner |
| Abstract |
Genome rearrangements underlie different human diseases including many cancers. Determining the rates at which genome rearrangements arise and isolating unique, independent genome rearrangements is critical to understanding the genes and pathways that prevent or promote genome rearrangements. Here, we describe quantitative S. cerevisiae genetic assays for measuring the rates of accumulating genome rearrangements including deletions, translocations, and broken chromosomes healed by de novo telomere addition that result in the deletion of two counter-selectable genes, CAN1 and URA3, placed in the nonessential regions of the S. cerevisiae genome. The assays also allow for the isolation of individual genome rearrangements for structural studies, and a method for analyzing genome rearrangements by next-generation DNA sequencing is provided. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 20% |
| Researcher | 3 | 20% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Professor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 3 | 20% |
| Computer Science | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Chemistry | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 3 | 20% |