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Mendeley readers
Chapter title |
High-Resolution Mapping of Modified DNA Nucleobases Using Excision Repair Enzymes
|
---|---|
Chapter number | 6 |
Book title |
Genome Instability
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7306-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Monica Ransom, D. Suzi Bryan, Jay R. Hesselberth |
Abstract |
Modification of DNA nucleobases has a profound effect on genome function. We developed a method that maps the positions of the modified DNA nucleobases throughout genomic DNA. This method couples in vitro nucleobase excision with massively parallel DNA sequencing to determine the location of modified DNA nucleobases with single base precision. This protocol was used to map uracil incorporation and UV photodimers in DNA, and a modification of the protocol has been used to map sparse modification events in cells. The Excision-seq protocol is broadly applicable to a variety of base modifications for which an excision enzyme is available. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 28% |
Researcher | 6 | 21% |
Professor | 3 | 10% |
Student > Master | 2 | 7% |
Student > Bachelor | 1 | 3% |
Other | 2 | 7% |
Unknown | 7 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 31% |
Agricultural and Biological Sciences | 4 | 14% |
Immunology and Microbiology | 3 | 10% |
Chemistry | 3 | 10% |
Physics and Astronomy | 2 | 7% |
Other | 1 | 3% |
Unknown | 7 | 24% |