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Cellular Quiescence

Overview of attention for book
Cover of 'Cellular Quiescence'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches
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    Chapter 2 An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts
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    Chapter 3 Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining
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    Chapter 4 Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells
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    Chapter 5 Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System
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    Chapter 6 Determination of Histone 2B–Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells
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    Chapter 7 Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation
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    Chapter 8 Cell Cycle Analysis by Mass Cytometry
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    Chapter 9 Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells
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    Chapter 10 Identifying Quiescent Stem Cells in Hair Follicles
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    Chapter 11 Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells
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    Chapter 12 Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence
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    Chapter 13 Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells
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    Chapter 14 Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation
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    Chapter 15 A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment
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    Chapter 16 Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis
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    Chapter 17 Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assays and RNA Immunoprecipitation (RIP)
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    Chapter 18 Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells
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    Chapter 19 Genome-Wide Identification of Transcription Factor-Binding Sites in Quiescent Adult Neural Stem Cells
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    Chapter 20 Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling
Attention for Chapter 13: Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells
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  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

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Chapter title
Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells
Chapter number 13
Book title
Cellular Quiescence
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7371-2_13
Pubmed ID
29030821
Book ISBNs
978-1-4939-7370-5, 978-1-4939-7371-2
Authors

Chun Shik Park, H. Daniel Lacorazza

Abstract

Hematopoietic stem cells (HSCs) represent an important target cell population in bone marrow transplantation, cell and gene therapy applications, and the development of leukemia models for research. Because the hematopoietic progeny carries the genetic information of HSCs and replenishes the blood and immune system, corrective gene transfer into HSCs provides an ideal therapeutic approach for many monogenic hematological diseases and a useful tool for studies of HSC function and blood formation in normal and malignant hematopoiesis. However, the efficiency of gene transfer into HSCs has been limited by several features of viral vectors, viral titer, methods of viral transduction, and the property of stem cell quiescence. In this chapter, we describe the production of retrovirus using murine stem cell virus (MSCV)-based retroviral vectors and purification and transduction of murine HSCs.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Researcher 2 25%
Student > Bachelor 1 13%
Student > Master 1 13%
Other 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 25%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Nursing and Health Professions 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Economics, Econometrics and Finance 1 13%
Other 1 13%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2017.
All research outputs
#14,956,881
of 23,006,268 outputs
Outputs from Methods in molecular biology
#4,728
of 13,160 outputs
Outputs of similar age
#255,644
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#508
of 1,498 outputs
Altmetric has tracked 23,006,268 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,254 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.

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