| Chapter title |
Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence
|
|---|---|
| Chapter number | 12 |
| Book title |
Cellular Quiescence
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7371-2_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7370-5, 978-1-4939-7371-2
|
| Authors |
Kai Yang, Hongbo Chi |
| Abstract |
Naïve T cells are in a quiescent state under homeostasis but respond to antigen stimulation by exiting from quiescence and entering the cell cycle. Appropriate regulation of quiescence is crucial for maintaining T cell homeostasis at steady state and initiating proper T cell responses to antigen stimulation. Emerging evidence indicates that signaling by mechanistic target of rapamycin (mTOR) plays a central role in the control of T cell quiescence and antigen-induced exit from quiescence through coordinating immune signals, cellular metabolic programs, and cell cycle machinery. The mTOR-dependent regulation of quiescence has also been implicated in the differentiation and function of memory T cells. In this chapter, we describe techniques to assess quiescent state of naïve T cells under steady state and exit from quiescence upon TCR stimulation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 14% |
| Researcher | 1 | 14% |
| Student > Doctoral Student | 1 | 14% |
| Unknown | 4 | 57% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Unknown | 5 | 71% |