Chapter title |
An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts
|
---|---|
Chapter number | 2 |
Book title |
Cellular Quiescence
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7371-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7370-5, 978-1-4939-7371-2
|
Authors |
Mithun Mitra, Linda D. Ho, Hilary A. Coller |
Abstract |
Cellular quiescence is a reversible mode of cell cycle exit that allows cells and organisms to withstand unfavorable stress conditions. The factors that underlie the entry, exit, and maintenance of the quiescent state are crucial for understanding normal tissue development and function as well as pathological conditions such as chronic wound healing and cancer. In vitro models of quiescence have been used to understand the factors that contribute to quiescence under well-controlled experimental conditions. Here, we describe an in vitro model of quiescence that is based on neonatal human dermal fibroblasts. The fibroblasts are induced into quiescence by antiproliferative signals, contact inhibition, and serum-starvation (mitogen withdrawal). We describe the isolation of fibroblasts from skin, methods for inducing quiescence in isolated fibroblasts, and approaches to manipulate the fibroblasts in proliferating and quiescent states to determine critical regulators of quiescence. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 70 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 19% |
Researcher | 12 | 17% |
Student > Master | 11 | 16% |
Student > Bachelor | 6 | 9% |
Student > Doctoral Student | 3 | 4% |
Other | 8 | 11% |
Unknown | 17 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 25 | 36% |
Agricultural and Biological Sciences | 9 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 6% |
Medicine and Dentistry | 3 | 4% |
Unspecified | 2 | 3% |
Other | 8 | 11% |
Unknown | 19 | 27% |