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Glucose Transport

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Cover of 'Glucose Transport'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris
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    Chapter 2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
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    Chapter 3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies
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    Chapter 4 GLUT Characterization Using Frog Xenopus laevis Oocytes
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    Chapter 5 Glucose Uptake in Heterologous Expression Systems
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    Chapter 6 Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer
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    Chapter 7 Glucose Transport Activity Measured in Giant Vesicles
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    Chapter 8 Design, Synthesis, and Evaluation of GLUT Inhibitors
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    Chapter 9 Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level
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    Chapter 10 A Growth-Based Screening System for Hexose Transporters in Yeast
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    Chapter 11 Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique
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    Chapter 12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking
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    Chapter 13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay
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    Chapter 14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence
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    Chapter 15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes
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    Chapter 16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery
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    Chapter 17 Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay
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    Chapter 18 Tracking GLUT2 Translocation by Live-Cell Imaging
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    Chapter 19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis
Attention for Chapter 4: GLUT Characterization Using Frog Xenopus laevis Oocytes
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Chapter title
GLUT Characterization Using Frog Xenopus laevis Oocytes
Chapter number 4
Book title
Glucose Transport
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7507-5_4
Pubmed ID
Book ISBNs
978-1-4939-7506-8, 978-1-4939-7507-5
Authors

Wentong Long, Debbie O’Neill, Chris I. Cheeseman

Abstract

Xenopus laevis oocytes are a useful heterologous expression system for expressing glucose transporters (GLUTs) and examining their functions. In this chapter, we provide a detailed protocol on oocyte extraction and preparation for GLUT9 protein expression. Furthermore, we describe the determination of GLUT9 overexpression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate kinetics by radioisotopes as well as two-microelectrode voltage clamping techniques.

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Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 50%
Researcher 1 50%
Readers by discipline Count As %
Nursing and Health Professions 1 50%
Medicine and Dentistry 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2017.
All research outputs
#18,578,649
of 23,011,300 outputs
Outputs from Methods in molecular biology
#7,962
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Outputs of similar age
#330,510
of 442,319 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,498 outputs
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