↓ Skip to main content
What is this page? Embed badge

Glucose Transport

Overview of attention for book
Cover of 'Glucose Transport'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris
  3. Altmetric Badge
    Chapter 2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
  4. Altmetric Badge
    Chapter 3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies
  5. Altmetric Badge
    Chapter 4 GLUT Characterization Using Frog Xenopus laevis Oocytes
  6. Altmetric Badge
    Chapter 5 Glucose Uptake in Heterologous Expression Systems
  7. Altmetric Badge
    Chapter 6 Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer
  8. Altmetric Badge
    Chapter 7 Glucose Transport Activity Measured in Giant Vesicles
  9. Altmetric Badge
    Chapter 8 Design, Synthesis, and Evaluation of GLUT Inhibitors
  10. Altmetric Badge
    Chapter 9 Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level
  11. Altmetric Badge
    Chapter 10 A Growth-Based Screening System for Hexose Transporters in Yeast
  12. Altmetric Badge
    Chapter 11 Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique
  13. Altmetric Badge
    Chapter 12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking
  14. Altmetric Badge
    Chapter 13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay
  15. Altmetric Badge
    Chapter 14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence
  16. Altmetric Badge
    Chapter 15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes
  17. Altmetric Badge
    Chapter 16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery
  18. Altmetric Badge
    Chapter 17 Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay
  19. Altmetric Badge
    Chapter 18 Tracking GLUT2 Translocation by Live-Cell Imaging
  20. Altmetric Badge
    Chapter 19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis
Attention for Chapter 2: Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

twitter
3 X users

Readers on

mendeley
17 Mendeley
Summary X
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
Chapter number 2
Book title
Glucose Transport
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7507-5_2
Pubmed ID
29218514
Book ISBNs
978-1-4939-7506-8, 978-1-4939-7507-5
Authors

Dong Deng, Nieng Yan, Deng, Dong, Yan, Nieng

Abstract

Overexpression, purification, and crystallization of eukaryotic membrane proteins represent a major challenge for structural biology. In recent years, we have solved the crystal structures of the human glucose transporters GLUT1 in the inward-open conformation at 3.17 Å resolution and GLUT3 in the outward-open and occluded conformations at 2.4 and 1.5 Å resolutions, respectively. Structural elucidation of these transporters in three distinct functional states reveal the molecular basis for the alternating access transport cycle of this prototypal solute carrier family. It established the molecular foundation for future dynamic and kinetic investigations of these GLUTs, and will likely facilitate structure-based ligand development. In this chapter, we present the detailed protocols of recombinant protein expression, purification, and crystallization of GLUT1 and GLUT3, which may help the pursuit of structural elucidation of other eukaryotic membrane proteins.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 35%
Student > Ph. D. Student 6 35%
Student > Master 2 12%
Student > Doctoral Student 1 6%
Student > Bachelor 1 6%
Other 1 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 53%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Agricultural and Biological Sciences 2 12%
Medicine and Dentistry 1 6%
Neuroscience 1 6%
Other 1 6%
Unknown 1 6%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2017.
All research outputs
#14,369,953
of 23,011,300 outputs
Outputs from Methods in molecular biology
#4,225
of 13,157 outputs
Outputs of similar age
#240,454
of 442,319 outputs
Outputs of similar age from Methods in molecular biology
#432
of 1,498 outputs
Altmetric has tracked 23,011,300 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,319 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.

This page is provided by Altmetric.