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Glucose Transport

Overview of attention for book
Cover of 'Glucose Transport'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris
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    Chapter 2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
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    Chapter 3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies
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    Chapter 4 GLUT Characterization Using Frog Xenopus laevis Oocytes
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    Chapter 5 Glucose Uptake in Heterologous Expression Systems
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    Chapter 6 Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer
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    Chapter 7 Glucose Transport Activity Measured in Giant Vesicles
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    Chapter 8 Design, Synthesis, and Evaluation of GLUT Inhibitors
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    Chapter 9 Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level
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    Chapter 10 A Growth-Based Screening System for Hexose Transporters in Yeast
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    Chapter 11 Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique
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    Chapter 12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking
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    Chapter 13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay
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    Chapter 14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence
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    Chapter 15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes
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    Chapter 16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery
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    Chapter 17 Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay
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    Chapter 18 Tracking GLUT2 Translocation by Live-Cell Imaging
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    Chapter 19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis
Attention for Chapter 10: A Growth-Based Screening System for Hexose Transporters in Yeast
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Chapter title
A Growth-Based Screening System for Hexose Transporters in Yeast
Chapter number 10
Book title
Glucose Transport
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7507-5_10
Pubmed ID
Book ISBNs
978-1-4939-7506-8, 978-1-4939-7507-5
Authors

Eckhard Boles, Mislav Oreb

Abstract

As the simplest eukaryotic model system, the unicellular yeast Saccharomyces cerevisiae is ideally suited for quick and simple functional studies as well as for high-throughput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Student > Ph. D. Student 3 20%
Researcher 2 13%
Student > Doctoral Student 1 7%
Student > Bachelor 1 7%
Other 1 7%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 40%
Agricultural and Biological Sciences 3 20%
Chemical Engineering 1 7%
Engineering 1 7%
Unknown 4 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2017.
All research outputs
#20,454,971
of 23,011,300 outputs
Outputs from Methods in molecular biology
#9,941
of 13,157 outputs
Outputs of similar age
#378,156
of 442,319 outputs
Outputs of similar age from Methods in molecular biology
#1,193
of 1,498 outputs
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So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.