Chapter title |
RareCyte® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis
|
---|---|
Chapter number | 15 |
Book title |
Circulating Tumor Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7144-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7143-5, 978-1-4939-7144-2
|
Authors |
Jackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre, Jay Tzucker, Jennifer Chow, Brennan Enright, Eric P. Kaldjian, Stilwell, Jackie L., Varshavskaya, Paulina, Werbin, Jeffrey L., Nordberg, Joshua J., Ramirez, Arturo B., Quarre, Steve, Tzucker, Jay, Chow, Jennifer, Enright, Brennan, Kaldjian, Eric P. |
Abstract |
The CytePicker module built into the RareCyte CyteFinder instrument allows researchers to easily retrieve individual cells from microscope slides for genomic analyses, including array CGH, targeted sequencing, and next-generation sequencing. Here, we describe the semiautomated retrieval of CTCs from the blood processed by AccuCyte (see Chapter 13) and amplification of genomic DNA so that molecular analysis can be performed. |
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