Chapter title |
Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability
|
---|---|
Chapter number | 2 |
Book title |
Circulating Tumor Cells
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7144-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7143-5, 978-1-4939-7144-2
|
Authors |
Emily S. Park, Simon P. Duffy, Hongshen Ma, Park, Emily S., Duffy, Simon P., Ma, Hongshen |
Abstract |
Circulating tumor cells (CTCs) have been implicated as the seeds of cancer metastasis and therefore have the potential to provide significant prognostic and diagnostic values. Here, we describe a procedure for separating CTCs from whole blood based on size and deformability using the microfluidic ratchet device. This device leverages the ratcheting motion of single cells created as they are deformed through funnel-shaped constrictions using oscillatory flow in order to divert cells based on differences in size and deformability. Subsequent methods for CTC identification and enumeration using immunofluorescence after separation are also described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 27% |
Student > Master | 3 | 27% |
Student > Bachelor | 1 | 9% |
Other | 1 | 9% |
Student > Postgraduate | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Engineering | 3 | 27% |
Computer Science | 1 | 9% |
Unknown | 3 | 27% |