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Mendeley readers
| Chapter title |
Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry
|
|---|---|
| Chapter number | 3 |
| Book title |
Peptidomics
|
| Published by |
Humana Press, New York, NY, February 2018
|
| DOI | 10.1007/978-1-4939-7537-2_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7536-5, 978-1-4939-7537-2
|
| Authors |
Kazuki Sasaki, Takashi Tsuchiya, Tsukasa Osaki |
| Abstract |
Media conditioned by cultured cells represent an excellent source rich in endogenous peptides. Unbiased mass spectrometric analysis of the constituent peptides provides an opportunity to look into proteolytic events such as bioactive peptide processing, membrane protein ectodomain shedding, or even regulated intramembrane proteolysis. If conducted on a large scale, peptidomics has the potential to pinpoint primary cleavage sites. Here a method is described for isolating peptides from cultured cell conditioned media before mass spectrometry analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 33% |
| Student > Doctoral Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Agricultural and Biological Sciences | 1 | 17% |
| Immunology and Microbiology | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |