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Peptidomics

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Cover of 'Peptidomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Origins, Technological Development, and Applications of Peptidomics
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    Chapter 2 Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics
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    Chapter 3 Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry
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    Chapter 4 Mass Spectrometric Identification of Endogenous Peptides
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    Chapter 5 Bioinformatics for Prohormone and Neuropeptide Discovery
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    Chapter 6 Substrate Capture Assay Using Inactive Oligopeptidases to Identify Novel Peptides
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    Chapter 7 Non-targeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS
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    Chapter 8 Quantitative Peptidomics: General Considerations
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    Chapter 9 Quantitative Peptidomics with Isotopic and Isobaric Tags
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    Chapter 10 Quantitative Peptidomics Using Reductive Methylation of Amines
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    Chapter 11 Metabolic Labeling to Quantify Drosophila Neuropeptides and Peptide Hormones
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    Chapter 12 Data Preprocessing, Visualization, and Statistical Analyses of Nontargeted Peptidomics Data from MALDI-MS
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    Chapter 13 Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity
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    Chapter 14 Mass Spectrometry Based Immunopeptidomics for the Discovery of Cancer Neoantigens
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    Chapter 15 Milk Peptidomics to Identify Functional Peptides and for Quality Control of Dairy Products
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    Chapter 16 Neuropeptidomic Analysis of Zebrafish Brain
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    Chapter 17 Identification, Quantitation, and Imaging of the Crustacean Peptidome
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    Chapter 18 Identification of Endogenous Neuropeptides in the Nematode C. elegans Using Mass Spectrometry
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    Chapter 19 EndoProteoFASP as a Tool to Unveil the Peptidome-Protease Profile: Application to Salivary Diagnostics
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    Chapter 20 Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
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    Chapter 21 Identification of Components in Frog Skin Secretions with Therapeutic Potential as Antidiabetic Agents
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    Chapter 22 High-Accuracy Mass Spectrometry Based Screening Method for the Discovery of Cysteine Containing Peptides in Animal Venoms and Toxins
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    Chapter 23 Analysis of the Snake Venom Peptidome
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    Chapter 24 Identification of Peptides in Spider Venom Using Mass Spectrometry
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    Chapter 25 Single Cell Peptidomics: Approach for Peptide Identification by N-Terminal Peptide Derivatization
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    Chapter 26 Peptidomic Identification of Cysteine-Rich Peptides from Plants
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    Chapter 27 Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss
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    Chapter 28 The Bright Future of Peptidomics
Attention for Chapter 27: Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss
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Chapter title
Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss
Chapter number 27
Book title
Peptidomics
Published by
Humana Press, New York, NY, February 2018
DOI 10.1007/978-1-4939-7537-2_27
Pubmed ID
Book ISBNs
978-1-4939-7536-5, 978-1-4939-7537-2
Authors

Igor Fesenko, Regina Khazigaleeva, Vadim Govorun, Vadim Ivanov

Abstract

Here, we report our approach to peptidomic analysis of the plant object which led to structure elucidation of the title peptides. P. patens samples were extracted under conditions preventing proteolytic digestion by endogenous proteases. The extracts were fractionated on size exclusion columns and the peptide fractions subjected to LC-MS/MS analysis. Mass spectra datasets were analyzed for the presence of peptides derived from the proteins encoded by the moss genome. Experimental details are given for each step, selected chromatograms and mass-spectra are presented in figures.

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Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 29%
Unspecified 1 14%
Student > Doctoral Student 1 14%
Professor 1 14%
Student > Master 1 14%
Other 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Computer Science 1 14%
Unspecified 1 14%
Other 0 0%