| Chapter title |
Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells
|
|---|---|
| Chapter number | 19 |
| Book title |
Mitophagy
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/7651_2017_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7749-9, 978-1-4939-7750-5
|
| Authors |
Yamashita, Shun-ichi, Kanki, Tomotake, Shun-ichi Yamashita, Tomotake Kanki |
| Abstract |
Mitochondrial quality and quantity are not only regulated by mitochondrial fusion and fission but also by mitochondria degradation. Mitophagy, an autophagy specific for damaged or unnecessary mitochondria, is believed to be an important pathway for mitochondrial homeostasis. To date, several stimuli are known to induce mitophagy. Some of these stimuli, however, including hypoxia, iron depletion, and nitrogen starvation, induce mild mitophagy, which is difficult to detect through decreased mitochondrial mass. Recently, we have clearly detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of hypoxia-induced and iron depletion-induced mitophagy using mito-Keima-expressed cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 43% |
| Professor | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Neuroscience | 2 | 29% |
| Chemistry | 1 | 14% |
| Unknown | 2 | 29% |