| Chapter title |
Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells
|
|---|---|
| Chapter number | 20 |
| Book title |
Mitophagy
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/7651_2017_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7749-9, 978-1-4939-7750-5
|
| Authors |
Fujimaki, Motoki, Saiki, Shinji, Sasazawa, Yukiko, Ishikawa, Kei-Ichi, Imamichi, Yoko, Sumiyoshi, Katsuhiko, Hattori, Nobutaka, Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi, Nobutaka Hattori |
| Abstract |
Both PINK1 and parkin are the responsible genes (PARK6 and PARK2, respectively) for familial early-onset Parkinson's disease (PD). Several lines of evidences have suggested that mitochondrial dysfunction would be associated with PD pathogenesis. Lewy body, one of PD pathological hallmarks, contains alpha-synuclein, a familial PD (PARK1/4)-gene product, which is eliminated by macroautophagy, while PINK1 and parkin coordinately mediate mitophagy (hereafter called as PINK1/parkin-mediated mitophagy) reported firstly by Youle's group. The mitochondrial quality control system is specific for elimination of damaged mitochondria especially in the loss of mitochondrial membrane potential induced by treatment with mitochondrial uncoupler like CCCP or FCCP. In this chapter, we summarized immunocytochemical methods to monitor the PINK1/parkin-mediated mitophagy using cultured cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Researcher | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 4 | 40% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Arts and Humanities | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 2 | 20% |