You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Validation of a Chemiluminescence Immunoassay for Measuring Amyloid-β in Human Blood Plasma
|
---|---|
Chapter number | 7 |
Book title |
Biomarkers for Alzheimer’s Disease Drug Development
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7704-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7703-1, 978-1-4939-7704-8
|
Authors |
Jonathan Vogelgsang, Jens Wiltfang, Hans W. Klafki |
Abstract |
The technical performance of immunological assays and their suitability for the intended use should be carefully validated before implementation in research, clinical studies or routine. We describe here the evaluation of a sandwich electrochemiluminescence immunoassay for measuring total Amyloid-β levels in human blood plasma as an example of a laboratory protocol for a partial "fit for purpose" assay performance validation. We tested two different assay protocols and addressed impact of sample dilution, parallelism, intra- and inter-assay variance, lower limit of quantification, lower limit of detection, and analytical spike recoveries. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Other | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 29% |
Neuroscience | 2 | 29% |
Immunology and Microbiology | 1 | 14% |
Unknown | 2 | 29% |