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Mendeley readers
| Chapter title |
Imaging DNA Repair After UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture
|
|---|---|
| Chapter number | 18 |
| Book title |
3D Sponge-Matrix Histoculture
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7745-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7743-7, 978-1-4939-7745-1
|
| Authors |
Shinji Miwa, Robert M. Hoffman, Miwa, Shinji, Hoffman, Robert M. |
| Abstract |
DNA damage repair in response to UVC irradiation was imaged in cancer cells growing in Gelfoam® histoculture. UVC-induced DNA damage repair was imaged with green fluorescent protein (GFP) fused to the DNA damage response (DDR)-related binding protein 53BP1 in MiaPaCa-2 human pancreatic cancer cells. Three-dimensional Gelfoam® histocultures and confocal imaging enabled 53BP1-GFP nuclear foci to be observed within 1 h after UVC irradiation, indicating the onset of DNA damage repair response. Induction of UV-induced 53BP1-GFP focus formation was limited up to a depth of 40 μm in Gelfoam® histoculture of MiaPaCa-2 cells, indicating this was the depth limit of UVC irradiation. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 50% |
| Researcher | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 1 | 50% |
| Unknown | 1 | 50% |