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The Mitotic Exit Network

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Cover of 'The Mitotic Exit Network'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Regulation of Mitotic Exit in Saccharomyces cerevisiae
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    Chapter 2 Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression
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    Chapter 3 Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining
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    Chapter 4 Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators
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    Chapter 5 Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release
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    Chapter 6 In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP
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    Chapter 7 Analysis of Protein–Protein Interactions Between MEN Components
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    Chapter 8 A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity
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    Chapter 9 Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain
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    Chapter 10 A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets
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    Chapter 11 Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast
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    Chapter 12 The Mitotic Exit Network
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    Chapter 13 Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)
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    Chapter 14 Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies
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    Chapter 15 Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays
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    Chapter 16 Analysis of the Functionality of the Mitotic Checkpoints
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    Chapter 17 Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions
  19. Altmetric Badge
    Chapter 18 Studying the Role of the Mitotic Exit Network in Cytokinesis
  20. Altmetric Badge
    Chapter 19 Hippo Signaling in Mitosis: An Updated View in Light of the MEN Pathway
Attention for Chapter 12: The Mitotic Exit Network
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Chapter title
The Mitotic Exit Network
Chapter number 12
Book title
The Mitotic Exit Network
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6502-1_12
Pubmed ID
Book ISBNs
978-1-4939-6500-7, 978-1-4939-6502-1
Authors

Guo, Zhiang, Segal, Marisa, Zhiang Guo, Marisa Segal

Abstract

Mitotic exit is determined by multiple spatial and temporal cues from the spindle poles and the two compartments in a dividing yeast cell-the mother and the bud. These signals are ultimately integrated by the activation of the mitotic exit network (MEN) to promote persistent release of Cdc14 from the nucleolus. Live imaging analysis using fluorescent protein tags is invaluable to dissect this critical decision-making trigger. Here, we present protocols for routine yeast live cell microscopy applicable to this problem.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Researcher 2 25%
Other 1 13%
Student > Postgraduate 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Medicine and Dentistry 2 25%
Agricultural and Biological Sciences 1 13%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2018.
All research outputs
#18,480,433
of 22,899,952 outputs
Outputs from Methods in molecular biology
#7,927
of 13,134 outputs
Outputs of similar age
#310,530
of 420,455 outputs
Outputs of similar age from Methods in molecular biology
#691
of 1,074 outputs
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