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Mendeley readers
| Chapter title |
Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining
|
|---|---|
| Chapter number | 3 |
| Book title |
The Mitotic Exit Network
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6502-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6500-7, 978-1-4939-6502-1
|
| Authors |
Marta Muñoz-Barrera, Fernando Monje-Casas, Muñoz-Barrera, Marta, Monje-Casas, Fernando |
| Abstract |
The budding index and the morphology of the spindle and the nucleus are excellent markers for the analysis of the progression through the different stages of the cell cycle in Saccharomyces cerevisiae. Here, we describe a protocol to evaluate the budding index in this model organism using phase contrast microscopy. We also describe an indirect immunofluorescence method designed for the visualization of microtubules and the nucleus in S. cerevisiae. Finally, we explain how both methodologies can be used in order to analyze cell cycle progression in the budding yeast. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Master | 1 | 10% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 3 | 30% |
| Unknown | 3 | 30% |