Chapter title |
Tumor Suppressor Analysis in CML
|
---|---|
Chapter number | 8 |
Book title |
Chronic Myeloid Leukemia
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4011-0_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4009-7, 978-1-4939-4011-0
|
Authors |
Oliver Herrmann, Mirle Schemionek, Herrmann, Oliver, Schemionek, Mirle |
Abstract |
Retroviral models have tremendously contributed to our understanding of CML development and have been indispensable for preclinical drug testing which facilitated the implementation of a targeted therapy. The retroviral insertion of Bcr-Abl into mice that are genetically depleted for a potential tumor suppressor is a tool to test for a specific gene function in Bcr-Abl disease. Here we describe how to generate a Bcr-Abl retrovirus that is subsequently used for infection of primary murine BM cells, which are genetically depleted for a potential tumor suppressor gene. We will suggest control experiments and outline further methods that are required to allow for assessment of disease development upon tumor suppressor knockout in CML. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Other | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Engineering | 1 | 33% |
Unknown | 1 | 33% |